Calculate solution dilution using C1V1 = C2V2. Find any missing variable: concentration or volume.
The Dilution Equation
The dilution formula C1×V1 = C2×V2 states that the amount of solute remains constant when you add solvent. C = concentration, V = volume.
Common Lab Dilutions
| Dilution | Ratio | Example |
|---|
| 1:10 | 1 part stock + 9 parts solvent | General lab dilution |
| 1:100 | 1 part + 99 parts | Bacterial cultures |
| 1:2 (serial) | Equal parts each step | Antibody titration |
How to Use This Dilution Calculator
Enter the starting concentration, desired final concentration, and one of the volumes (stock or final). The calculator determines the other volume and the dilution ratio.
Formula & How It Works
C1 Γ V1 = C2 Γ V2, where C1 = initial concentration, V1 = volume of stock, C2 = final concentration, V2 = final volume. Dilution ratio = V2 / V1.
Calculation Example
Diluting 10% bleach to 1% solution: C1=10%, C2=1%, want V2=1000 mL. V1 = (1 Γ 1000) / 10 = 100 mL. Mix 100 mL bleach with 900 mL water.
Expert Tips
Always add the concentrated solution to the diluent (solvent), not the reverse β especially with acids. For serial dilutions, prepare each step from the previous dilution, not the stock. Label everything clearly.